Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescence intensity resolution in flow systems.

M J McCutcheon, R G Miller

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Older age of childhood type 1 diabetes onset is associated with islet autoantibody positivity >30 years later: the Pittsburgh Epidemiology of Diabetes Complications Study.

    Diabetic medicine : a journal of the British Diabetic Association·2020
    Same author

    Improved XLT4 Agar: Small Addition of Peptone to Promote Stronger Production of Hydrogen-Sulfide by Salmonellae.

    Journal of food protection·2019
    Same author

    Improved XLT4 Agar: Small Addition of Peptone to Promote Stronger Production of Hydrogen-Sulfide by Salmonellae.

    Journal of food protection·2019
    Same author

    Evaluation of Two Isolation and Two Nonisolation Methods for Detecting Naturally Occurring Salmonellae from Broiler Flock Environmental Drag-Swab Samples.

    Journal of food protection·2019
    Same author

    Predictors of early renal function decline in adults with Type 1 diabetes: the Coronary Artery Calcification in Type 1 Diabetes and the Pittsburgh Epidemiology of Diabetes Complications studies.

    Diabetic medicine : a journal of the British Diabetic Association·2017
    Same author

    Modulation of the CO<sub>2</sub> fixation in dinickel azacryptands.

    Dalton transactions (Cambridge, England : 2003)·2017
    Same journal

    Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    ADAMTS1 Is Required for Ventral Abdominal Wall Closure.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    FGF2 Boost for Driving Forebrain Organoid Maturation Under Static Conditions.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    See all related articles

    Flow cytometry resolution depends on signal intensity. For dim signals, shot noise is limiting, while bright signals are affected by hydrodynamics and illumination stability. Photon statistics are key for intermediate signals.

    Area of Science:

    • Biotechnology
    • Analytical Chemistry
    • Cell Biology

    Background:

    • Flow cytometry is a powerful technique for analyzing single cells.
    • Fluorescence intensity resolution is critical for accurate cell characterization.
    • Factors limiting resolution in flow cytometry require detailed understanding.

    Purpose of the Study:

    • To identify and analyze the primary factors limiting fluorescence intensity resolution in flow cytometry.
    • To understand how signal intensity influences the dominant resolution-limiting factors.

    Main Methods:

    • Analysis of signal intensity-dependent limitations in a flow cytometer system.
    • Characterization of resolution limits for bright and weak fluorescent sources.
    • Investigation of photoelectron statistical variations (photon statistics) as a resolution determinant.

    Related Experiment Videos

    Main Results:

    • For bright signals (e.g., DNA stains), resolution is limited to ~3% coefficient of variation by factors like hydrodynamics and illumination stability.
    • For weak signals (e.g., cell-surface labels), resolution is limited by photomultiplier shot noise due to background light.
    • Intermediate signal brightness is primarily limited by photon statistics, emphasizing photon collection and detection efficiency.

    Conclusions:

    • Flow cytometry resolution is multifaceted, with different factors dominating at varying signal intensities.
    • Optimizing photon collection and detection efficiency is crucial for improving resolution, especially for intermediate signals.
    • Understanding these limitations enables better experimental design and instrument optimization for flow cytometry applications.