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Updated: Jul 23, 2025
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
Published on: February 4, 2017
A quantitative description of the compatibility of technetium-selective chromatographic technetium-99m separation
Jaewoong Jang1, Shun Sekimoto2, Tsutomu Ohtsuki2
1Department of Bioengineering, School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan; Isotope Science Center, University of Tokyo, 2-11-16 Yayoi, Bunkyo, Tokyo 113-0032, Japan.
Abstract:
Technetium-99m generators employing a technetium-selective stationary phase are a chromatographic instrument developed for use with 99Mo having low specific activity (LSA); particularly, 99Mo produced by electron accelerators. This paper presents a mathematical description of technetium-selective chromatographic (TSC) 99mTc separation and analyzes its compatibility with LSA 99Mo. We developed a theoretical formula for TSC 99mTc separation by discretizing its pertechnetate selectivity, and validated it using an electron linear accelerator and activated carbon-based TSC (AC-TSC) 99mTc generators. We confirmed that the activity concentration of 99mTc obtained from a TSC 99mTc generator can be calculated directly from its input 99Mo activity regardless of the 99Mo specific activity. The formula corroborates that TSC 99mTc separation is compatible with LSA 99Mo, and has a practical application in estimating the number of TSC 99mTc generators required for 99mTc demand of interest.
