Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The lepromin test in rhesus monkeys.

G B Baskin, B J Gormus, L N Martin

    International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
    |September 1, 1986
    PubMed
    Summary

    The lepromin test is effective in rhesus monkeys for leprosy diagnosis, but requires higher antigen concentrations. A 10X concentration of lepromin A is recommended for accurate results in monkey studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cancellation properties in Hall magnetohydrodynamics with a strong guide magnetic field.

    Physical review. E, Statistical, nonlinear, and soft matter physics·2014
    Same author

    Required energy for a laser radar system incorporating a fiber amplifier or an avalanche photodiode.

    Applied optics·2010
    Same author

    Application of bispectral speckle imaging to near-diffraction-limited imaging in the presence of unknown aberrations.

    Applied optics·2010
    Same author

    Liver abscess due to Friedlander's bacillus (K. pneumoniae) complicating adenocarcinoma of ampulla of Vater.

    American journal of clinical pathology·2010
    Same author

    Imaging thermal objects with photon-counting detectors.

    Applied optics·2010
    Same author

    Intermittency in the isotropic component of helical and nonhelical turbulent flows.

    Physical review. E, Statistical, nonlinear, and soft matter physics·2010

    Area of Science:

    • Veterinary Immunology
    • Mycobacterial Research
    • Primate Models in Disease

    Background:

    • The lepromin test is a diagnostic tool for cellular immunity in leprosy.
    • Its efficacy and optimal antigen concentration in non-human primates, specifically rhesus monkeys, remain to be fully elucidated.
    • Understanding immune responses in animal models is crucial for leprosy research.

    Purpose of the Study:

    • To evaluate the utility of the lepromin test in rhesus monkeys.
    • To determine the appropriate antigen concentration for lepromin testing in this species.
    • To correlate lepromin test results with experimental Mycobacterium leprae inoculation and disease status.

    Main Methods:

    • Rhesus monkeys were divided into control, experimentally induced leprosy, and M. leprae-inoculated (non-diseased) groups.

    Related Experiment Videos

  • Various concentrations of lepromin A, lepromin M, and purified inactivated M. leprae were used for skin testing.
  • Reactions were assessed and correlated with inoculation and disease status.

    • Main Results:

    • The lepromin test showed utility in rhesus monkeys, necessitating higher antigen concentrations than used in humans.
    • Control and lepromatous leprosy monkeys were lepromin negative.
    • Monkeys inoculated with M. leprae but without developed leprosy were lepromin positive.
    • Indeterminate leprosy cases showed intermediate reactions.
    • 10X lepromin A was identified as a useful preparation for rhesus monkey testing.

    Conclusions:

    • The lepromin test is a valuable tool for assessing cellular immunity in rhesus monkeys regarding Mycobacterium leprae.
    • Optimized antigen concentrations, specifically 10X lepromin A, are essential for reliable lepromin testing in this primate model.
    • Lepromin test results can differentiate between resistant, susceptible, and infected but non-diseased states in M. leprae-inoculated rhesus monkeys.