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X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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Related Experiment Video

Updated: Jul 23, 2025

Fixed Target Serial Data Collection at Diamond Light Source

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

A standard descriptor for fixed-target serial crystallography.

Robin L Owen¹, Daniele de Sanctis², Arwen R Pearson³

¹Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.

Acta Crystallographica. Section D, Structural Biology

|July 18, 2023

Summary

This summary is machine-generated.

A new standardized descriptor, not a device, is proposed to describe fixed targets for serial crystallography. This innovation aims to simplify data collection and enhance the exchange of fixed targets between synchrotron and X-ray free-electron laser (XFEL) sources.

Keywords:

fixed targets serial crystallography standardization

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Area of Science:

Crystallography
Structural Biology
X-ray Science

Background:

Fixed-target crystallography is crucial for serial crystallography at synchrotron and X-ray free-electron laser (XFEL) sources.
A wide variety of fixed targets exist, lacking standardization in design and dimensions.
The diversity of experimental needs makes a universal standard device impractical.

Purpose of the Study:

To propose a standardized descriptor for fixed targets in serial crystallography.
To facilitate data collection and the exchange of fixed targets between different facilities.
To improve compatibility and usability of fixed targets across various experimental setups.

Main Methods:

Development of a standardized descriptor in dictionary format.
The descriptor is designed to be readable by fixed-target beamline software.
The descriptor facilitates straightforward data collection from new fixed targets.

Main Results:

A simple, standardized descriptor is proposed as an alternative to a standardized device.
This descriptor enables beamline software to interpret and utilize new fixed targets.
Facilitates easier exchange and adoption of fixed targets across different sources.

Conclusions:

The proposed descriptor simplifies the integration of diverse fixed targets into serial crystallography workflows.
It enhances interoperability between different synchrotron and XFEL facilities.
This standardization benefits beamlines, researchers, and manufacturers by streamlining experimental setup and data acquisition.