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Inhibitory Effects of Wheat Sprouts Extract on RANKL-Induced Osteoclast Differentiation via Suppressing MAPK and
Bok Kyung Han1, Hyeock Yoon2, Kyeong Hoon Kim3
1Department of Food and Biotechnology and Korea University, Sejong, Korea.
Journal of Medicinal Food
|July 18, 2023
Summary
Wheat sprout extracts (SGG and ARH) effectively inhibit osteoclast differentiation and bone resorption without cytotoxicity. These natural compounds show potential for preventing bone disorders like osteoporosis.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Bone remodeling relies on osteoclasts (bone resorption) and osteoblasts (bone formation).
- Imbalances in bone remodeling contribute to disorders like osteoporosis.
- Current bone resorption inhibitors may have side effects, necessitating novel preventive strategies.
Purpose of the Study:
- To investigate the inhibitory effects of wheat sprout ethanolic extracts (Saegeumgang [SGG] and Arriheuk [ARH]) on osteoclast differentiation.
- To elucidate the mechanisms underlying the anti-osteoclastogenic effects of SGG and ARH.
- To assess the potential of SGG and ARH as functional foods or therapeutic agents for bone health.
Main Methods:
- Osteoclast differentiation was induced in RAW 264.7 cells using RANKL.
- Effects on osteoclast differentiation and bone resorption were evaluated via TRAP staining, pit formation assays, and TRAP activity assays.
- Gene and protein expression levels of osteoclast-specific markers were analyzed using RT-qPCR and Western blotting.
Main Results:
- SGG and ARH significantly suppressed RANKL-induced osteoclast differentiation in RAW 264.7 cells without exhibiting cytotoxicity.
- Both extracts dose-dependently reduced TRAP-positive cell counts, TRAP activity, and pit formation area.
- SGG and ARH inhibited the expression of key osteoclastogenesis-related genes, including RANK, NFATc1, cathepsin K, and TRAP.
Conclusions:
- Wheat sprout extracts SGG and ARH demonstrate potent inhibitory effects on osteoclast differentiation and activity.
- These extracts modulate critical molecular pathways involved in osteoclastogenesis.
- SGG and ARH hold promise as natural agents for managing bone-related disorders and promoting bone health.
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