Construction of DNA Bilayer Tiles and Arrays Using Circular DNA Molecules as Scaffolds

Area of Science:

• * Nanotechnology and Materials Science
• * Supramolecular Chemistry
• * Synthetic Biology

Background:

• * Two-dimensional (2D) tile self-assembly using oligonucleotides, like double crossovers (DX) and multi-arm junction (mAJ) tiles, is established.
• * The construction of three-dimensional (3D) oligonucleotide tiles remains challenging and less explored.
• * Developing efficient methods for 3D tile assembly is crucial for creating complex nanostructures.

Purpose of the Study:

• * To report the successful construction of novel bilayer tiles for 3D self-assembly.
• * To introduce and validate five E-tiling approaches for creating 3D nanostructures.
• * To demonstrate the assembly of porous nanotubes and planar microscale arrays using the developed tiles.

Main Methods:

• * Synthesis of two new bilayer tiles utilizing small circular 84mer oligonucleotides as scaffolds.
• * Design and application of five distinct E-tiling strategies for assembly.
• * Employing solution assembly for nanotube formation and surface-mediated assembly for planar arrays.

Main Results:

• * Achieved high yield in the construction of the new bilayer tiles.
• * Successfully fabricated porous nanotubes with micron-long dimensions in medium yield.
• * Demonstrated the creation of densely covered planar microscale arrays via surface-mediated assembly.

Conclusions:

• * The developed bilayer tiles and E-tiling approaches provide a viable route for constructing complex 3D nanostructures.
• * This work expands the capabilities of oligonucleotide self-assembly into the third dimension.
• * The ability to form both nanotubes and planar arrays opens possibilities for diverse nanoscale applications.