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MiR-128-3p promotes the progression of deep venous thrombosis through binding SIRT1

Jinan Han1, Wanjiang Hao2, Yanping Ma3

  • 1Department of Vascular Surgery, Hulunbuir People's Hospital, Hulunbuir, China.

Phlebology
|July 19, 2023
PubMed
Abstract

Insights

MicroRNA-128-3p (miR-128-3p) impacts deep venous thrombosis (DVT) by targeting SIRT1. Upregulated miR-128-3p in DVT patients suggests its potential as a diagnostic biomarker.

Area of Science:

  • Vascular Biology
  • Molecular Medicine
  • Biomarker Discovery

Background:

  • Deep venous thrombosis (DVT) is a significant vascular condition.
  • Understanding the molecular mechanisms underlying DVT is crucial for diagnosis and treatment.
  • MicroRNAs play regulatory roles in various cellular processes relevant to thrombosis.

Purpose of the Study:

  • To investigate the role of microRNA-128-3p (miR-128-3p) in the pathogenesis of deep venous thrombosis (DVT).
  • To explore the relationship between miR-128-3p and its potential target, silent information regulator sirtuin 1 (SIRT1).
  • To evaluate the diagnostic potential of miR-128-3p in DVT patients.

Main Methods:

  • Cellular assays including MTT, Transwell, and flow cytometry were employed.
  • Luciferase activity assays identified the interaction between miR-128-3p and SIRT1.
  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) measured miR-128-3p and SIRT1 mRNA levels.
  • Receiver operating characteristic (ROC) curve analysis assessed the predictive value of miR-128-3p in DVT.

Main Results:

  • Reduced miR-128-3p expression promoted human umbilical vein endothelial cell (HUVEC) proliferation and migration while inhibiting inflammation, apoptosis, and adhesion.
  • The effects of miR-128-3p on HUVECs were mediated through targeting SIRT1.
  • miR-128-3p was found to be upregulated in patients with DVT and showed significant diagnostic capability.

Conclusions:

  • Overexpression of miR-128-3p plays a role in DVT development.
  • miR-128-3p demonstrates potential as a diagnostic biomarker for DVT patients.
Keywords:
DVTHUVECSIRT1diagnosismiR-128-3p

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