Related Experiment Video
Updated: Jul 23, 2025

A Protocol for Collecting and Constructing Soil Core Lysimeters
Published on: June 6, 2016
Nutrient-cycling functional gene diversity mirrors phosphorus transformation during chicken manure composting
1National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
Abstract:
Elucidating ecological mechanism underlying phosphorus transformation mediated by phosphate-solubilizing bacteria (PSB) during manure composting is an important but rarely investigated subject. The research objective is to disentangle ecological functions of the inoculation of PSB Pseudomonas sp. WWJ-22 during chicken manure composting based on gene quantification and amplicon sequencing. There are large dynamic changes in phosphorus fractions, gene abundances, and bacterial community structure. The PSB addition notably increased available phosphorus from 0.29-0.89 g kg-1 to 0.49-1.39 g kg-1 and significantly affected phosphorus fractionation. The PSB inoculation significantly affected composition of nutrient-cycling functional genes (NCFGs), and notably influenced bacterial community composition and function. Compost bacteria showed significant phylogenetic signals in response to phosphorus fractions, and stochastic processes dominated bacterial community assembly. Results emphasized that PSB addition increased functional redundancy, phylogenetic conservatism, and stochasticity-dominated assembly of bacterial community. Overall, findings highlight NCFG diversity can be a bio-indicator to mirror phosphorus transformation.
Related Concept Videos
The Phosphorus Cycle
Environmental Applications of Microorganisms
Metabolism of Chemolithotrophs
The Roles of Bacteria and Fungi in Plant Nutrition
Microbial Nutrition
Diversity of Archaea I

