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Updated: Jul 23, 2025
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Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride
Published on: July 1, 2020
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Screen-Printed Silver/Silver Chloride Electrodes Inhibit Alcohol Oxidase Activity.
Bob M Lansdorp1, Peter Lamberg1, Rashad Hamid1
1Milo Sensors, Inc. Santa Barbara, CA, 93101, United States of America.
ECS Sensors Plus
|July 20, 2023
Summary
Screen-printed silver/silver chloride (Ag/AgCl) electrodes significantly reduce Alcohol Oxidase enzyme stability. This finding impacts the design of electrochemical biosensors relying on Ag/AgCl reference electrodes.
Area of Science:
- Electrochemistry
- Enzyme kinetics
- Biosensor technology
Background:
- Silver/silver chloride (Ag/AgCl) is a common reference electrode material in electrochemical biosensors.
- The stability of enzymes used in biosensors is critical for their performance and longevity.
Purpose of the Study:
- To investigate the effect of screen-printed Ag/AgCl ink on the stability of Alcohol Oxidase enzyme.
- To quantify the inhibition of enzymatic activity caused by Ag/AgCl electrodes.
Main Methods:
- An optical absorbance assay was employed to monitor enzyme activity.
- The stability of Alcohol Oxidase was compared in the presence and absence of Ag/AgCl electrodes.
Main Results:
- Ag/AgCl electrodes drastically reduced the halftime of Alcohol Oxidase activity from approximately one week to 10 hours.
- A strong inhibitory effect of the Ag/AgCl electrode on enzyme stability was observed.
Conclusions:
- Screen-printed Ag/AgCl ink negatively impacts Alcohol Oxidase enzyme stability.
- This interaction has significant implications for the development and application of enzymatic biosensors utilizing Ag/AgCl reference electrodes.
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