A visualized sensor based on layered double hydroxides with peroxidase-like activity for sensitive...

Area of Science:

• Biochemistry
• Materials Science
• Analytical Chemistry

Background:

• Acetylcholinesterase (AChE) is vital for neurotransmission, and its abnormal levels are linked to neurodegenerative diseases.
• Developing simple, sensitive platforms for AChE analysis is crucial for disease diagnosis and research.

Purpose of the Study:

• To construct a straightforward and highly sensitive colorimetric sensor for acetylcholinesterase (AChE) detection.
• To utilize the peroxidase-like activity of Ni/Co layered double hydroxides (Ni/Co LDHs) for AChE quantification.

Main Methods:

• Fabrication of Ni/Co LDHs with inherent peroxidase-like catalytic activity.
• Development of a colorimetric assay where Ni/Co LDHs catalyze OPD oxidation, inhibited by AChE.
• Modification of Ni/Co LDHs onto paper for a visual, paper-based analytical device.

Main Results:

• The Ni/Co LDHs exhibited significant peroxidase-like activity, catalyzing OPD to oxOPD.
• AChE presence inhibited this activity, with inhibition correlating to AChE concentration.
• The paper-based sensor achieved a sensitive detection limit of 6.6 μU mL⁻¹ for AChE.

Conclusions:

• A simple, equipment-free, and sensitive colorimetric sensor for AChE detection was successfully developed.
• The sensor, utilizing Ni/Co LDHs, demonstrates potential for on-site, real-time, and visualized AChE detection.
• This technology holds promise for applications in clinical diagnosis and biomedical fields.