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Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Stimuli-Responsive DNA-Based Hydrogels on Surfaces for Switchable Bioelectrocatalysis and Controlled Release of Loads
Michael Fadeev1, Gilad Davidson-Rozenfeld1, Zhenzhen Li1
1The Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
ACS Applied Materials & Interfaces
|July 21, 2023
Summary
Enzyme-loaded DNA hydrogels on electrodes offer tunable stiffness for controlled bioelectrocatalysis. This enables switchable glucose oxidase functions and triggered insulin release, advancing smart biosensor technology.
Area of Science:
- Biomaterials Science
- Bioelectrochemistry
- DNA Nanotechnology
Background:
- Stimuli-responsive hydrogels offer dynamic control over material properties.
- Enzyme-loaded hydrogels are crucial for biosensing and drug delivery applications.
- DNA-based hydrogels provide precise structural control and biocompatibility.
Purpose of the Study:
- To develop enzyme-loaded, stimuli-responsive DNA hydrogels for switchable bioelectrocatalytic functions.
- To demonstrate triggered control over hydrogel stiffness for tunable bioactivity.
- To achieve glucose concentration-controlled, potential-triggered insulin release from modified electrodes.
Main Methods:
- Assembly of glucose oxidase (GOx)-loaded DNA hydrogels on electrode surfaces.
- Utilizing pH-responsive i-motif and K+-ion/crown ether-responsive G-quadruplex DNA structures for crosslinking.
- Investigating hydrogel stiffness changes triggered by pH, K+ ions, and crown ether.
- Demonstrating potential-triggered, glucose-controlled insulin release using electron mediators or integrated matrices.
Main Results:
- Successfully assembled GOx-loaded, stimuli-responsive DNA hydrogels with tunable stiffness.
- Demonstrated switchable bioelectrocatalytic activity of GOx by altering hydrogel stiffness.
- Achieved triggered insulin release from hydrogel-modified electrodes in response to glucose concentration and electrical potential.
- Showcased two distinct hydrogel systems responsive to pH and K+ ions, respectively.
Conclusions:
- Enzyme-loaded DNA hydrogels with tunable stiffness provide a platform for switchable bioelectrocatalysis.
- The developed systems enable precise control over enzyme activity and drug release.
- This approach holds promise for advanced biosensors, drug delivery systems, and bioelectronic devices.
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