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Updated: Jul 22, 2025

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
Active peptides with hypoglycemic effect obtained from hemp (Cannabis sativa L) protein through identification,
Lei Cai1, Shengwen Wu1, Chenggang Jia2
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China.
Abstract:
Hemp (Cannabis sativa L) seeds are rich in proteins of high nutritional value, which makes the study of beneficial properties of hemp seed proteins and peptides, such as hypotensive and hypoglycemic effects, increasingly attractive. The present results confirm the good processability and stability of the hemp protein hydrolysate obtained by enzymatic hydrolysis of non-dehulled hemp seed meal (NDHM). Six peptides with potential hypoglycemic activity were obtained by ethanol-graded precipitation, Nano LC-Q-Orbitrap-MS/MS mass spectrometry, and computerized virtual screening. Further, validation experiments for in vitro synthesis showed that TGLGR, SPVI, FY, and FR exhibited good α-glucosidase inhibitory activity, respectively. Animal experiments showed that the hemp protein peptides modulated blood glucose and blood lipids in hyperglycemic rats. These results indicate that hemp protein peptides can reduce blood glucose levels in hyperglycemic rats, suggesting that hemp proteins may be a promising natural source for the prevention and treatment of hyperglycemia.
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