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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 22, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Clinical Cytometry for Platelets and Platelet Disorders.

Andrew L Frelinger¹, Benjamin E J Spurgeon¹

¹Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA 02115, USA.

Clinics in Laboratory Medicine

|July 22, 2023

Summary

This summary is machine-generated.

Flow cytometry offers valuable diagnostic tests for platelet disorders, including inherited conditions and antibody detection. Further research is needed to confirm the clinical utility of advanced flow cytometry methods for patient diagnosis and treatment monitoring.

Keywords:

Blood platelet disorders Flow cytometry Phenotyping Platelet activation Platelet function tests Thrombocytopenia

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Area of Science:

Hematology
Immunology
Clinical Diagnostics

Background:

Clinical flow cytometry is a valuable tool for diagnosing inherited and acquired platelet disorders.
Current flow cytometric methods can detect glycoprotein deficiencies, secretion defects, drug-induced thrombocytopenias, and antiplatelet antibodies.
Availability of these diagnostic tests remains limited in clinical settings.

Purpose of the Study:

To review the utility of clinical flow cytometry in the diagnosis of platelet disorders.
To highlight the potential of advanced multicolor flow cytometry and high-dimensional immunophenotyping for identifying novel platelet subsets.
To emphasize the need for studies evaluating new flow cytometric tests for patient diagnosis and therapy monitoring.

Main Methods:

Review of existing flow cytometric methodologies for platelet analysis.
Discussion of advanced techniques including multicolor flow cytometry and high-dimensional immunophenotyping.
Identification of specific platelet defects detectable by flow cytometry.

Main Results:

Flow cytometry can effectively detect various inherited and acquired platelet abnormalities.
Advanced multicolor flow cytometry enables the identification of previously unrecognized platelet subsets.
Pharmacodynamic inhibition by antiplatelet agents can be assessed using flow cytometry.

Conclusions:

Clinical flow cytometry represents a powerful diagnostic approach for platelet disorders.
Emerging high-dimensional immunophenotyping techniques offer new avenues for platelet subset identification.
Further clinical studies are essential to validate the diagnostic and therapeutic monitoring value of these advanced flow cytometry tests.