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Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
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Advances in Clinical Mass Cytometry.

Abhishek Koladiya1, Kara L Davis2

  • 1Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA.

Clinics in Laboratory Medicine
|July 22, 2023
PubMed
Summary

Mass cytometry (CyTOF) offers advanced immune cell profiling, detecting cellular diversity in health and disease. This technology enhances immune monitoring with high throughput and cost-effectiveness for clinical research applications.

Keywords:
Clinical researchCytometryHigh-dimensionalInfectious diseaseMass cytometryNeuroscienceProteinSignaling

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Area of Science:

  • Immunology
  • Biotechnology
  • Clinical Research

Background:

  • High-dimensional single-cell technologies reveal immune cell heterogeneity and functional diversity.
  • Mass cytometry (CyTOF) surpasses flow cytometry in immune monitoring due to multiplexing and minimal compensation.
  • CyTOF offers higher throughput and lower cost than other single-cell techniques.

Purpose of the Study:

  • Introduce CyTOF-based assays for immune cell profiling.
  • Detail methods for analyzing immune cell types and states.
  • Highlight applications of CyTOF in clinical research.

Main Methods:

  • Utilizing mass cytometry (CyTOF) for high-dimensional single-cell analysis.
  • Developing assays to profile diverse immune cell populations.
  • Leveraging CyTOF's capabilities for immune monitoring.

Main Results:

  • CyTOF enables detailed characterization of immune cell phenotypes.
  • Identification of cellular features associated with disease outcomes.
  • Demonstration of CyTOF's utility in clinical settings.

Conclusions:

  • CyTOF is a powerful tool for immune cell profiling.
  • CyTOF assays provide valuable insights into immune responses in health and disease.
  • This technology has significant applications in advancing clinical research.