Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BRD1 haploinsufficiency alters early neuronal programming and disrupts maturation in human induced glutamatergic neurons.

Research square·2026
Same author

BRD1 haploinsufficiency alters early neuronal programming and disrupts maturation in human induced glutamatergic neurons.

bioRxiv : the preprint server for biology·2025
Same author

Development of a biological at-risk volume using apparent diffusion coefficient for parotid-sparing radiation therapy planning.

BJR open·2025
Same author

MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity.

NPJ Parkinson's disease·2024
Same author

TMEFF1 is a neuron-specific restriction factor for herpes simplex virus.

Nature·2024
Same author

The HIF transcription network exerts innate antiviral activity in neurons and limits brain inflammation.

Cell reports·2024

Related Experiment Video

Updated: Jul 22, 2025

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
10:25

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Published on: January 23, 2018

21.5K

Protocol for generating reproducible miniaturized controlled midbrain organoids.

Muwan Chen1, Jonathan Christos Niclis2, Mark Denham1

  • 1Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, 8000C Aarhus, Denmark; Department of Biomedicine, Aarhus University, 8000C Aarhus, Denmark.

STAR Protocols
|July 23, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a protocol for creating reproducible, non-necrotic miniaturized controlled midbrain organoids (MiCOs). This method enhances stem cell research for disease modeling and drug screening.

Keywords:
NeuroscienceOrganoids

More Related Videos

A High-Throughput Platform for Culture and 3D Imaging of Organoids
07:42

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Published on: October 14, 2022

2.8K
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
10:16

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

5.6K

Related Experiment Videos

Last Updated: Jul 22, 2025

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
10:25

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Published on: January 23, 2018

21.5K
A High-Throughput Platform for Culture and 3D Imaging of Organoids
07:42

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Published on: October 14, 2022

2.8K
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
10:16

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

5.6K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Midbrain organoids are crucial for studying neurodevelopmental and neurodegenerative diseases.
  • Existing protocols often struggle with reproducibility, size control, and central necrosis.
  • Developing standardized methods is essential for reliable research.

Purpose of the Study:

  • To present a robust protocol for generating miniaturized controlled midbrain organoids (MiCOs).
  • To ensure reproducible size and cellular composition without a necrotic core.
  • To facilitate applications in compound screening and disease modeling.

Main Methods:

  • Human pluripotent stem cells were maintained and passaged.
  • MiCOs were generated using AggreWell™400 technology.
  • Organoids were cultured on an EB-Disk™ on an orbital shaker, avoiding Matrigel and spinner flasks.

Main Results:

  • Reproducible generation of miniaturized controlled midbrain organoids achieved.
  • Organoids exhibited consistent size and cellular composition.
  • Absence of a necrotic center and prevention of organoid fusion were confirmed.

Conclusions:

  • The presented protocol offers a reliable method for MiCO generation.
  • This standardized approach is suitable for high-throughput compound screening.
  • MiCOs are a valuable tool for in vitro disease modeling of the midbrain.