Chronic posterior pituitary lobectomy: prolonged elevation of plasma prolactin and interruption of cyclicity

We previously reported that the posterior pituitary dopaminergic system participates in the inhibition of prolactin (PRL) secretion in both male and lactating female rats. However, posterior pituitary lobectomy (Lobex) of urethane-anesthetized cycling rats resulted in an elevation in plasma PRL for a short time only. This raises a question regarding the importance of input from the posterior pituitary to the control of PRL secretion during the estrous cycle. The objectives of this study were to examine the chronic effects of Lobex on plasma PRL levels in conscious rats and to determine whether the absence of input from the posterior pituitary interferes with estrous cyclicity. Lobex or sham lobectomy were performed under Brevital anesthesia in estrous rats. Blood was collected from jugular cannula at hourly intervals on the day of surgery and at 09.00, 13.00, and 17.00 h during the following 4 days. Daily water consumption and vaginal cyclicity were monitored for 14 and 20 days, respectively. Within 2 h after Lobex, the plasma PRL levels rose 3- to 4-fold and remained elevated for 3 days before declining to near control levels on the 4th day. None of the Lobex rats resumed cyclicity within 3-4 days, 50% had an interruption of cyclicity for 4-10 days, and the remainder were noncyclic for more than 11 days. Upon resumption of cyclicity, Lobex rats had 11.3 +/- 0.4 oviductal ova which is within the normal range for intact ovulating rats.(ABSTRACT TRUNCATED AT 250 WORDS)