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Precise Analysis of Nanoparticle Size Distribution in TEM Image.

Shan Zhang1, Chao Wang2

  • 1School of Materials Science and Engineering, Institute of Materials Science and Devices, Suzhou University of Science and Technology, 99 Xuefu Road, Suzhou 215011, China.

Methods and Protocols
|July 25, 2023
PubMed
Summary

This study presents an efficient method for nanoparticle size distribution analysis using ImageJ and Origin software. It offers a standardized approach to overcome manual measurement limitations in transmission electron microscopy (TEM).

Keywords:
ImageJnanoparticlesize distributiontransmission electron microscope

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Nanoparticle size distribution is crucial for synthesis, optimization, and application.
  • Manual measurement of nanoparticles from transmission electron microscope (TEM) images is time-consuming and prone to errors.
  • Standardized methods are needed for accurate nanoparticle size analysis, especially for irregularly shaped particles.

Purpose of the Study:

  • To develop an efficient and standardized method for nanoparticle size distribution analysis.
  • To overcome the limitations of manual measurement techniques.
  • To provide a template for accurate nanoparticle characterization.

Main Methods:

  • Utilized basic ImageJ software (version 1.53t) for analyzing nanoparticle size from TEM images.
  • Employed Origin software for processing the collected size data.
  • Applied the method to determine the size distribution of typical silica nanoparticles.

Main Results:

  • Achieved rapid and accurate size distribution analysis of silica nanoparticles.
  • Demonstrated the effectiveness of ImageJ and Origin software for this purpose.
  • Established a reproducible workflow for nanoparticle characterization.

Conclusions:

  • The developed method provides an efficient and standardized approach for nanoparticle size distribution analysis.
  • This technique can serve as a paradigm for future nanoparticle characterization studies.
  • Automating size analysis enhances accuracy and reduces time compared to manual methods.