Partitioning Recovery of Natural Pigments from Spirulina platensis

In the last few decades, the recovery of high-added value compounds with high food potential using microalgae as feedstock has been one of the main challenges for both research and industry. This study provides a simple, affordable, and cost-saving approach for the selective recovery of C-phycocyanin (C-PC), chlorophyll a and carotenoids from Spirulina platensis biomass by using biocompatible and industrially approved solvents (such as CaCl₂ and ethanol). The concentration and yield of each pigment in the liquid extract have been spectrophotometrically detected, whereas the decolorized protein-rich biomass has been analyzed by CIELab parameters. The most concentrated (566.4 μg/mL) and food-grade C-PC extract (purity index 0.7) was obtained by applying a biomass/solvent ratio (1:10) for the first round of extraction (20 min), followed by a second round at 1:5 using CaCl₂ 1.5% (w/v) aqueous solution. Additionally, the same trial enabled the production of the brightest decolorized protein-rich biomass (L* = 46.2), characterized by a yellow-orange tonality (h° values = 81.3°).