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Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Imaging Studies III: Computed Tomography01:27

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DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Interface self-referenced dynamic full-field optical coherence tomography.

Tual Monfort1,2, Salvatore Azzollini1, Tasnim Ben Yacoub1

  • 1Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France.

Biomedical Optics Express
|July 27, 2023
PubMed
Summary
This summary is machine-generated.

Interface Self-Referenced (iSR) Dynamic Full-Field Optical Coherence Tomography (D-FFOCT) overcomes artefacts and vibration sensitivity. This novel D-FFOCT configuration enables clear imaging of biological samples, including flat cell cultures.

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Area of Science:

  • Biomedical optics
  • Cellular imaging
  • Optical coherence tomography

Background:

  • Dynamic full-field optical coherence tomography (D-FFOCT) is a label-free, non-invasive imaging technique for subcellular structures.
  • Standard D-FFOCT faces challenges with fringe artefacts near reflective surfaces and vibration sensitivity.
  • These limitations hinder high-resolution imaging in certain biological samples.

Purpose of the Study:

  • To introduce interface Self-Referenced (iSR) D-FFOCT as an alternative imaging modality.
  • To address and overcome the artefacts and vibration sensitivity issues inherent in conventional D-FFOCT.
  • To demonstrate the capability of iSR D-FFOCT for imaging delicate biological specimens.

Main Methods:

  • Developed interface Self-Referenced (iSR) D-FFOCT configuration.
  • Utilized the sample coverslip as a defocused reference arm.
  • Applied the technique to image 2D fibroblast cell cultures.

Main Results:

  • Successfully eliminated fringe artefacts common in standard D-FFOCT.
  • Significantly reduced sensitivity to environmental vibrations.
  • Achieved clear, high-resolution imaging of 2D fibroblast cell cultures.
  • Demonstrated the utility of iSR D-FFOCT for imaging flat mammalian cells.

Conclusions:

  • iSR D-FFOCT offers a robust solution for label-free, non-invasive imaging.
  • The method enhances image quality by mitigating artefacts and vibration sensitivity.
  • This technique is particularly suitable for imaging sensitive and delicate biological samples like cell cultures.