Permittivity-Based Microparticle Classification by the Integration of Impedance Cytometry and Microwave Resonators

Permittivity of microscopic particles can be used as a classification parameter for applications in materials and environmental sciences. However, directly measuring the permittivity of individual microparticles has proven to be challenging due to the convoluting effect of particle size on capacitive signals. To overcome this challenge, a sensing platform is built to independently obtain both the geometric and electric size of a particle, by combining impedance cytometry and microwave resonant sensing in a microfluidic chip. This way the microwave signal, which contains both permittivity and size effects, can be normalized by the size information provided by impedance cytometry to yield an intensive parameter that depends only on permittivity. The technique allows to differentiate between polystyrene and soda lime glass microparticles-below 22 µm in diameter-with more than 94% accuracy, despite their similar sizes and electrical characteristics. Furthermore, it is shown that the same technique can be used to differentiate between normal healthy cells and fixed cells of the same geometric size. The technique offers a potential route for targeted applications such as environmental monitoring of microplastic pollution or quality control in pharmaceutical industry.