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Updated: Jul 21, 2025

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

[Conditional optimization and methodological evaluation of liquid protein microarray in detecting cystatin C].

Chaoqun Sun¹, Jiangping Niu¹, Jiyong Yin¹

¹Key Laboratory of Trace Element Nutrition, National Health Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research

|July 27, 2023

Summary

This summary is machine-generated.

This study optimized conditions for detecting cystatin C (CysC) using liquid protein microarray. The method demonstrated good accuracy and precision, establishing a reliable platform for CysC analysis.

Keywords:

conditional optimization cystatin C liquid protein microarray methodological evaluation

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Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Context:

Cystatin C (CysC) is a biomarker for kidney function.
Accurate and efficient detection methods are crucial for clinical diagnostics.
Liquid protein microarrays offer a high-throughput platform for biomarker detection.

Purpose:

To optimize detection conditions for cystatin C (CysC) using a liquid protein microarray.
To perform a comprehensive methodological evaluation of the optimized CysC detection assay.
To establish a reliable and sensitive method for CysC quantification.

Summary:

Optimized antibody concentrations (26.6 μg/mL-1:800) for CysC detection via liquid protein microarray.
Established a regression equation (y=-3.315x^2+283.04x+160.89, R^2=0.9921) with a reportable range of 0.26-3784.04 ng/mL.
Demonstrated good assay performance with high precision (within-run 3.54%-4.03%) and accuracy (relative bias 5.81%), though hemoglobin showed interference.

Impact:

Provides an optimized and validated liquid protein microarray method for cystatin C detection.
Enhances the potential for high-throughput screening and clinical application of CysC measurements.
Contributes to the development of advanced diagnostic tools for kidney function assessment.