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Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
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Related Experiment Video

Updated: Jul 21, 2025

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Published on: July 28, 2022

The Septin Cytoskeleton is Required for Plasma Membrane Repair.

M Isabella Prislusky, Jonathan Gt Lam, Viviana Ruiz Contreras

Biorxiv : the Preprint Server for Biology

|July 28, 2023

Summary

Septin 7 (SEPT7) is crucial for mammalian cell plasma membrane repair, forming cytoskeletal scaffolds that recruit repair molecules like annexin A2 (ANXA2) to reseal injuries.

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Author Spotlight: Exploring Plasma Membrane Repair Mechanisms with Innovative Thermoplasmonic Puncturing

Author Spotlight: Exploring Plasma Membrane Repair Mechanisms with Innovative Thermoplasmonic Puncturing

Published on: January 19, 2024

Area of Science:

Cell Biology
Cytoskeletal Dynamics
Membrane Repair Mechanisms

Background:

Mammalian cells face constant mechanical and biochemical stress, leading to plasma membrane injuries.
Effective plasma membrane repair is vital for cellular homeostasis and survival.
Existing repair mechanisms involve proteins like annexin A2 (ANXA2), but novel candidates are continually sought.

Approach:

A silencing RNA screen identified novel plasma membrane repair proteins, focusing on septin 7 (SEPT7).
Experimental approaches, including super-resolution microscopy, investigated SEPT7's role in repair.
Cellular responses to pore-forming toxins and mechanical wounding were analyzed.

Key Points:

Septin 7 (SEPT7) is essential for plasma membrane repair following toxin-induced or mechanical injury.
Upon injury, SEPT7 reorganizes into Ca2+-dependent subplasmalemmal domains containing F-actin, myosin II, and ANXA2.
These SEPT7-dependent domains are critical for efficient membrane resealing.

Conclusions:

The septin cytoskeleton acts as a scaffold, promoting the formation of plasma membrane repair domains.
SEPT7 plays a key structural role in remodeling the membrane and recruiting ANXA2 for repair.
This study reveals a novel model for septin-mediated plasma membrane repair.