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Multichannel Resonant Acoustic Rheometry System for Rapid and Efficient Quantification of Human Plasma Coagulation.
Christina Hendren1, Weiping Li1, Jan P Stegemann1
1Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Research Square
|July 28, 2023
Summary
A new multichannel Resonant Acoustic Rheometry (mRAR) system enables real-time, simultaneous monitoring of multiple plasma samples. This ultrasound-based technique accurately assesses blood coagulation, correlating with International Normalized Ratio (INR) levels.
Area of Science:
- Biomedical Engineering
- Materials Science
- Hematology
Background:
- Plasma coagulation is a complex process vital for hemostasis.
- Accurate, real-time monitoring of coagulation is crucial for clinical diagnostics and treatment.
- Existing methods may lack efficiency or real-time, multi-sample capabilities.
Approach:
- Developed a multichannel Resonant Acoustic Rheometry (mRAR) system using 4 custom ultrasound transducers (5.0 MHz).
- Implemented synchronized electronic driving for simultaneous, real-time monitoring of multiple small-volume plasma samples.
- Tested mRAR with normal and Coumadin-treated plasma (varying INR) using kaolin or tissue factor triggers.
Key Points:
- mRAR system successfully monitored dynamic changes throughout the entire coagulation process.
- Identified key coagulation parameters: clotting time, clotting speed, and clot mechanical properties.
- Results demonstrated a clear correlation between mRAR-derived parameters and Coumadin dosage/INR levels.
Conclusions:
- The mRAR system is feasible for rapid, efficient, and accurate characterization of plasma coagulation.
- This multi-sample capability significantly enhances the throughput for coagulation assessment.
- mRAR offers a promising non-contact ultrasound-based method for quantitative coagulation analysis.
