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Updated: Jul 21, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

A microfluidic strategy to capture antigen-specific high affinity B cells.

Ahmed M Alhassan¹, Venktesh S Shirure¹, Jean Luo²

¹Department of Biomedical Engineering, University of California, Davis.

Biorxiv : the Preprint Server for Biology

|July 28, 2023

Summary

This summary is machine-generated.

A new microfluidic method rapidly assesses B cell antigen binding affinity under force. This approach can identify high-affinity B cells for immune status assessment and clinical applications.

Keywords:

affinity avidity cell separation immunology

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Published on: February 6, 2018

Area of Science:

Immunology
Biophysics
Microfluidics

Background:

Current methods for assessing B cell responses and antibody affinity are often costly, time-consuming, and do not account for the influence of force on binding interactions.
Recent research suggests that mechanical force can modulate B cell receptor (BCR)-antigen binding, impacting immune status assessment.

Purpose of the Study:

To develop and validate a microfluidic strategy for assessing antigen-specific B cell receptor (BCR) binding affinity under physiologically relevant forces.
To demonstrate the utility of this method in enriching for high-affinity B cells and correlating BCR-antigen interactions with antibody solution affinity.

Main Methods:

A laminar flow microfluidic chamber was engineered with immobilized influenza A hemagglutinin antigen.
Five hemagglutinin-specific hybridomas were analyzed for antigen-specific BCR binding affinity across a force range of 65 to 650 pN.
The method assessed BCR binding under varying shear forces and measured bound lifetime.

Main Results:

Increasing shear force and longer bound lifetimes effectively enriched for antigen-specific, high-affinity B cells.
The measured affinity of membrane-bound BCRs in the microfluidic chamber showed strong correlation with the affinity of corresponding antibodies in solution.
The microfluidic strategy demonstrated rapid assessment of BCR-antigen binding properties.

Conclusions:

A microfluidic approach can rapidly and effectively assess BCR-antigen binding affinity and identify high-affinity B cells.
This technology holds potential for assessing functional immune status from peripheral B cells.
The method offers a cost-effective means for identifying B cells as sources for therapeutic antibody development.