Ultrasensitive Nonenzymatic Real-Time Hydrogen Peroxide Monitoring Using Gold Nanoparticle-Decorated Titanium Dioxide...

An amperometric enzyme-free hydrogen peroxide (H₂O₂) sensor was developed by catalytically stabilizing active gold nanoparticles (Au NPs) of 4-5 nm on a porous titanium dioxide nanotube (TiO₂ NTs) electrode. The Au NPs were homogeneously distributed on anatase TiO₂ NTs with an outer diameter of ~102 nm, an inner diameter of ~60 nm, and a wall of thickness of ~40 nm. The cyclic voltammogram of the composite electrode showed a pair of redox peaks characterizing the electrocatalytic reduction of H₂O₂. The entrapping of Au NPs on TiO₂ NTs prevented aggregation and facilitated good electrical conductivity and electron transfer rate, thus generating a wide linear range, a low detection limit of ~104 nM, and high sensitivity of ~519 µA/mM, as well as excellent selectivity, reproducibility, repeatability, and stability over 60 days. Furthermore, excellent recovery and relative standard deviation (RSD) were achieved in real samples, which were tap water, milk, and Lactobacillus plantarum bacteria, thereby verifying the accuracy and potentiality of the developed nonenzymatic sensor.