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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
Published on: October 13, 2023
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Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells
Valber A Pedrosa1, Kangfu Chen2, Thomas J George3
1Institute of Bioscience of Botucatu, Sao Paulo State University-Unesp, Botucatu 18603-560, Brazil.
Biosensors
|July 28, 2023
Summary
This study presents an efficient liquid biopsy method for capturing circulating tumor cells (CTCs) using gold nanoparticles on a microfluidic device. The system achieves high-efficiency CTC isolation in under 120 minutes, aiding cancer diagnosis.
Area of Science:
- Biomedical Engineering
- Oncology
- Nanotechnology
Background:
- Liquid biopsy is crucial for cancer diagnosis and monitoring.
- Current methods for capturing circulating tumor cells (CTCs) require improvement for efficiency and speed.
Purpose of the Study:
- To develop an efficient and rapid system for isolating CTCs.
- To enhance CTC capture using gold nanoparticles integrated with a microfluidic platform.
Main Methods:
- Integration of gold nanoparticles with antibodies onto a lateral filter array (LFA) microdevice.
- Optimization of the CTC capture system using pancreatic tumor cell lines.
- Demonstration of the platform for isolating CTCs from metastatic pancreatic cancer patients.
Main Results:
- Achieved high-efficiency CTC capture (90% ± 3.2%) within 120 minutes.
- Successfully isolated CTCs from patients with metastatic pancreatic cancer.
- Developed a method for creating functionalized surfaces for selective CTC capture.
Conclusions:
- The developed microfluidic platform with gold nanoparticles offers an efficient and rapid method for CTC isolation.
- This technology enhances selective CTC capture for downstream biological assays.
- The system shows promise for improving liquid biopsy applications in cancer detection and monitoring.
