Spatial Omics Sequencing Based on Microfluidic Array Chips

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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Published on: March 13, 2017

Jianyu Shi¹, Yating Pan¹, Xudong Liu¹

¹State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, College of Control Science and Engineering, Zhejiang University, Hangzhou 310000, China.

|July 28, 2023

Abstract:

Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces the development of spatial omics sequencing based on microfluidic array chips and describes barcoding strategies using various microfluidic designs with simplicity and efficiency. At the same time, the pros and cons of each strategy are compared. Moreover, commercialized solutions for spatial profiling are also introduced. In the end, the future perspective of spatial omics sequencing and research directions are discussed.

Keywords:

in situ capture and sequencing microfluidic array chip single-cell sequencing spatial multi-omics sequencing spatial transcriptomics

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