Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stiff matrix-induced KRTAP2-3 expression suppresses ciliogenesis via actin tension-driven chromatin remodeling.

Cell death & disease·2026

Same author

Nanocollision promotes locomotion of dendritic cells for tumor therapy.

Science advances·2026

Same author

Complete Mitochondrial Genomic Characteristics and Phylogenetic Analysis of the Medicinal Plant <i>Peperomia leptostachya</i>.

Genes·2026

Same author

NO<sub>3</sub><sup>-</sup>-Mediated Solvent Immobilization in Medium-Concentration Ether-Based Electrolytes: Enabling High-Voltage Lithium Metal Batteries with Wide Temperature Ranges.

ACS applied materials & interfaces·2025

Same author

A directional self-priming continuous-driven compartmentalized microfluidic chip for multiplexed pathogen detection.

The Analyst·2025

Same author

Enhanced Optoelectronic Performance of Two-Dimensional Organic Semiconductor Phototransistors Using Polystyrene Microsphere-Based Light-Trapping Structures.

ACS applied materials & interfaces·2025

Same journal

A Coumarin-Based Probe for Sequential ON-OFF-ON Detection of Cu<sup>2+</sup> and Biothiols: Naked-Eye Detection, Smartphone RGB Readout and In Vivo Imaging.

Biosensors·2026

Same journal

Electropolymerized Molecularly Imprinted Polymers Supported on Carbon-Based Materials for (Bio)sensing: Direct and Indirect Detection Strategies.

Biosensors·2026

Same journal

Progress in (Photo)electrochemical Biosensors for the Detection of Amyloid-Beta Oligomer.

Biosensors·2026

Same journal

Design and Simulation of Lamotrigine Intermittent Release from a Subcutaneous Implant with an Enzymatic Biosensor Based on Clinical Data.

Biosensors·2026

Same journal

Prediction of Chronic Kidney Disease Based on Simulated Serum Analysis by Vibrational Spectroscopy.

Biosensors·2026

Same journal

AI/ML-Assisted SERS Biosensing for Biomolecular Detection: From Direct Spectral Response to Integrated Diagnostic Systems.

Biosensors·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 21, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Spatial Omics Sequencing Based on Microfluidic Array Chips.

Jianyu Shi¹, Yating Pan¹, Xudong Liu¹

¹State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, College of Control Science and Engineering, Zhejiang University, Hangzhou 310000, China.

|July 28, 2023

Summary

This summary is machine-generated.

Spatial omics sequencing recovers lost spatial information from single-cell sequencing. This review covers microfluidic technologies, barcoding strategies, and commercial solutions for spatial profiling.

Keywords:

in situ capture and sequencing microfluidic array chip single-cell sequencing spatial multi-omics sequencing spatial transcriptomics

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Related Experiment Videos

Last Updated: Jul 21, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Traditional single-cell sequencing often loses crucial spatial context.
Spatial profiling technologies are emerging to address this limitation.
Recent advancements have significantly improved the resolution and simplicity of spatial profiling.

Purpose of the Study:

To review the development of spatial omics sequencing using microfluidic array chips.
To describe and compare various barcoding strategies for spatial profiling.
To introduce commercialized spatial profiling solutions and discuss future perspectives.

Main Methods:

Review of literature on spatial omics sequencing technologies.
Analysis of microfluidic designs for spatial barcoding.
Comparison of different barcoding strategies' simplicity and efficiency.
Overview of commercially available spatial profiling platforms.

Main Results:

Spatial omics sequencing based on microfluidic chips offers high resolution and simplicity.
Various barcoding strategies exist, each with distinct advantages and disadvantages.
Several commercial solutions for spatial profiling are now available.

Conclusions:

Spatial omics sequencing is a rapidly advancing field with significant potential.
Microfluidic technologies and efficient barcoding are key drivers of progress.
Continued innovation in spatial profiling will enhance our understanding of biological systems.