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Selected Technical Aspects of Molecular Allergy Diagnostics.

Kinga Lis1, Zbigniew Bartuzi1

  • 1Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-168 Bydgoszcz, Poland.

Current Issues in Molecular Biology
|July 28, 2023
PubMed
Summary

Allergy diagnosis involves complex laboratory tests, including specific immunoglobulin E (sIgE) measurements. Molecular allergy diagnosis analyzes sIgE to allergen components, offering advanced insights for better patient care.

Keywords:
ALEXISACallergen componentallergen extractallergyanti-CCDmolecular diagnosticsspecific IgE

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Area of Science:

  • Clinical immunology
  • Diagnostic laboratory science
  • Allergology

Background:

  • Allergic disease diagnosis is a multi-stage process utilizing various tools.
  • In vitro diagnostics (IVD), including laboratory tests, are crucial for allergy diagnosis.
  • Standard tests measure serum specific immunoglobulin E (sIgE) to allergens or allergen components.

Purpose of the Study:

  • To present technical aspects of modern allergy diagnostics.
  • To focus on advancements in molecular allergy diagnostics.
  • To review different immunochemical test methods for sIgE measurement.

Main Methods:

  • Review of in vitro diagnostic (IVD) test kits for allergy.
  • Analysis of singleplex (one-parameter) and multiplex (multi-parameter) immunochemical assays.
  • Discussion of serum specific immunoglobulin E (sIgE) measurement techniques.

Main Results:

  • Various IVD test kits are available, differing in methodology and allergen profiles.
  • Molecular allergy diagnosis quantifies sIgE to specific allergen components (molecules).
  • Both singleplex and multiplex assays are employed for sIgE detection.

Conclusions:

  • Modern allergy diagnostics leverage advanced IVD technologies.
  • Molecular allergy diagnostics provide detailed insights into sensitization profiles.
  • Technical aspects of various assays are critical for accurate allergy diagnosis.