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Related Concept Videos

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Lectin-Seq: A method to profile lectin-microbe interactions in native communities.

Robert L McPherson1,2, Christine R Isabella1,2, Rebecca L Walker3

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Science Advances
|July 28, 2023
PubMed
Summary
This summary is machine-generated.

Human lectins are key to innate immunity and microbiota regulation. This study reveals distinct binding profiles for mannose-binding lectin (MBL) and intelectin-1 (hItln1) in the human gut microbiome.

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Area of Science:

  • Immunology
  • Microbiology
  • Biochemistry

Background:

  • Soluble human lectins are crucial for innate immunity.
  • Lectins are suspected to influence host-resident microbiota, but their specific targets are not well understood.

Purpose of the Study:

  • To profile human lectin recognition within native microbial communities.
  • To understand the specific microbial species bound by lectins in the human gut.

Main Methods:

  • Development of a novel method, Lectin-Seq, for profiling lectin recognition.
  • Application of Lectin-Seq to human fecal microbiota samples.
  • Analysis of binding patterns for mannose-binding lectin (MBL) and intelectin-1 (hItln1).

Main Results:

  • Both MBL and hItln1 bound a significant percentage (10-20%) of fecal samples.
  • MBL exhibited selective binding to a small subset of common human-associated species.
  • hItln1 displayed broader binding across a diverse range of lower-abundance microbial species.

Conclusions:

  • Human lectins MBL and hItln1 possess markedly different microbial recognition profiles.
  • These findings highlight distinct roles for lectins in modulating the human gut microbiome composition and function.