Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Myasthenia Gravis: Overview and Treatment01:20

Myasthenia Gravis: Overview and Treatment

1.6K
Myasthenia gravis is a neuromuscular transmission disorder characterized by weakness and increased fatigability of skeletal muscles. It is an autoimmune disease affecting approximately one in 2000 people, where antibodies against the α1 subunit of nicotinic acetylcholine receptors are produced.
These antibodies interfere with the function of the nicotinic receptors in three ways: by binding to the receptor and disrupting acetylcholine binding; by causing cross-linking of receptors which...
1.6K
Alzheimer's Disease: Treatment01:22

Alzheimer's Disease: Treatment

219
Alzheimer's Disease (AD), a neurodegenerative disorder, is pathologically identified by amyloid plaques and neurofibrillary tangles composed of tau protein. AD pharmacotherapy aims to manage cognitive symptoms, delay disease progression, and treat behavioral symptoms. The treatment is primarily symptomatic and palliative, with no definitive disease-modifying therapy available. Cholinesterase inhibitors, including donepezil (Aricept), rivastigmine (Exelon), and galantamine (Razadyne), are...
219
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

14.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
14.5K
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

12
Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
12
Chronic Pancreatitis II: Collaborative Care01:29

Chronic Pancreatitis II: Collaborative Care

113
The management of chronic pancreatitis is multifaceted, involving a comprehensive approach that includes thorough assessment, diagnostic testing, and a variety of management strategies.
Assessment:
113
Indirect-Acting Cholinergic Agonists: Pharmacological Actions01:30

Indirect-Acting Cholinergic Agonists: Pharmacological Actions

716
Indirect-acting cholinergic agonists, also known as anticholinesterases, exert their pharmacological effects by enhancing cholinergic transmission in various body parts, including the neuromuscular junction, autonomic cholinergic synapses, and the brain.
At the neuromuscular junction, these agents work by inhibiting the breakdown of acetylcholine, allowing it to remain bound to the receptor and bind to nearby receptors. This process leads to repetitive firing of the endplate, causing muscle...
716

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diving exposure and pulmonary stress.

The Journal of physiology·2026
Same author

Pushing athletic performance boundaries with anodal transcranial direct current stimulation: an exploratory, pilot, randomized controlled study on strength and inflammation in elite weightlifters.

BMC sports science, medicine & rehabilitation·2026
Same author

Imbalanced cortisol/BDNF signals and oxidative stress underscore temporary cognitive impairment in deep divers after in water/surface decompression: proof of concept.

European journal of applied physiology·2026
Same author

The Proteomics-Based Stratification of Obese Subjects Allows for a Second Selective Level Beyond Gender Classification.

International journal of molecular sciences·2026
Same author

Effects of Transcutaneous Spinal Direct Current Stimulation on Cognitive and Psychological Outcomes in Multiple Sclerosis: A Preliminary Case Series.

Biomedicines·2026
Same author

Oxy-Inflammatory Profile of Finishers and No-Finishers in an Extreme Ultra-Endurance Trail Race: The 866 km Transpyrénéa.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Jul 21, 2025

Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination
09:38

Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination

Published on: September 12, 2016

12.3K

Chelation Therapy Associated with Antioxidant Supplementation Can Decrease Oxidative Stress and Inflammation in

Alessandra Vezzoli1, Simona Mrakic-Sposta1, Cinzia Dellanoce1

  • 1Institute of Clinical Physiology, National Research Council (IFC-CNR), Piazza Ospedale Maggiore 3, 20159 Milano, Italy.

Antioxidants (Basel, Switzerland)
|July 29, 2023
PubMed
Summary

Antioxidant chelation therapy with EDTA and micronutrients reduced oxidative stress and inflammation in multiple sclerosis (MS) patients. This treatment improved the balance of oxy-inflammation, potentially protecting against axonal damage and demyelination in MS.

Keywords:
EDTAEPRROScytokinesneurodegenerationneurotoxicityoxidative damagethiols’ redox status

More Related Videos

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.8K
Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
03:35

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research

Published on: June 28, 2024

1.4K

Related Experiment Videos

Last Updated: Jul 21, 2025

Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination
09:38

Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination

Published on: September 12, 2016

12.3K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.8K
Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research
03:35

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research

Published on: June 28, 2024

1.4K

Area of Science:

  • Neuroimmunology
  • Oxidative Stress Research
  • Clinical Nutrition

Background:

  • Multiple sclerosis (MS) is characterized by axonal damage and demyelination, linked to an imbalance in oxy-inflammation.
  • Oxidative stress and inflammation play critical roles in the pathogenesis of MS.

Purpose of the Study:

  • To investigate the efficacy of antioxidant treatment using calcium disodium ethylenediaminetetracetic acid (EDTA) chelation therapy combined with a micronutrient complex in MS patients.
  • To assess the impact of this treatment on oxidative stress markers, antioxidant capacity, and inflammatory status in MS.

Main Methods:

  • Recruited 20 MS patients and 20 healthy controls (CTR).
  • Measured plasma reactive oxygen species (ROS) production and total antioxidant capacity (TAC) using Electron Paramagnetic Resonance.
  • Assessed antioxidant system activities, urinary oxidative stress biomarkers, plasma cytokines (TNFα, IL-1β, IL-6), sICAM levels, nitric oxide (NO) catabolism, and transthyretin (TTR) concentration.

Main Results:

  • MS patients exhibited higher ROS production, oxidative damage, inflammatory biomarkers, and NO metabolites, with lower TAC compared to CTR.
  • Treatment significantly down-regulated pro-inflammatory markers (sICAM1, TNF-α, IL6) and biomarkers of lipid peroxidation and DNA damage in MS patients.
  • The treatment led to a decrease in ROS production and an increase in antioxidant capacity, shifting towards a more reduced thiols' status.

Conclusions:

  • Antioxidant chelation therapy with EDTA and micronutrients demonstrates a protective effect in MS patients.
  • The treatment appears to restore the balance of oxy-inflammation by reducing oxidative stress and enhancing antioxidant capacity.
  • This approach holds potential for managing MS by mitigating oxidative damage and inflammation.