Radical-Driven Methane Formation in Humans Evidenced by Exogenous Isotope-Labeled DMSO and Methionine.

Area of Science:

• Biochemistry
• Human Physiology
• Oxidative Stress Research

Background:

• Methane (CH4) is endogenously produced in organisms and may influence cellular signaling.
• Its role in human physiology and non-microbial production in mammals remains unclear.
• Potential therapeutic benefits of CH4 supplementation for diseases like inflammation and injury have been proposed.

Purpose of the Study:

• To provide the first evidence of non-microbial methane (CH4) formation in the human body.
• To investigate the cellular origin of endogenous CH4 using labeled precursors.
• To explore the potential physiological and stress-related roles of CH4 in humans.

Main Methods:

• Utilized isotopically labeled (2H and 13C) dimethyl sulfoxide (DMSO) and methionine as carbon precursors.
• Administered labeled DMSO topically, orally, and to blood samples from a human volunteer.
• Monitored stable isotope ratios of methane (CH4) in experimental samples.

Main Results:

• Demonstrated the conversion of methyl groups from labeled DMSO and methionine into methane (CH4).
• Confirmed the formation of isotopically labeled CH4 across all administration routes.
• Provided proof of principle for endogenous, non-microbial CH4 generation in humans.

Conclusions:

• Endogenous methane (CH4) is formed in the human body via a non-microbial, radical-driven process.
• Further research is warranted to elucidate CH4's physiological functions and bioactivity.
• Methane (CH4) may serve as a biomarker for oxidative stress and immune responses.