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Related Experiment Video

Updated: Jul 21, 2025

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

Discovery of Bactericidal Proteins from Staphylococcus Phage Stab21 Using a High-Throughput Screening Method.

Ellisiv Nyhamar^1,2, Paige Webber¹, Olivia Liong¹

¹Department of Bacteriology and Immunology, Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00290 Helsinki, Finland.

Antibiotics (Basel, Switzerland)

|July 29, 2023

Summary

Researchers screened 96 Hypothetical Proteins of Unknown Function (HPUFs) from a Staphylococcus phage. Five HPUFs showed bactericidal activity against E. coli, highlighting potential new antimicrobial strategies.

Keywords:

Hypothetical Proteins of Unknown Function (HPUFs)Staphylococcus aureus antimicrobial resistance bacteriophages cross-species toxicity

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Last Updated: Jul 21, 2025

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Area of Science:

Microbiology
Genomics
Biotechnology

Background:

Antimicrobial resistance necessitates novel antibiotic discovery.
Bacteriophages represent a promising, yet underexplored, source of antimicrobials.
Hypothetical Proteins of Unknown Function (HPUFs) in phages are largely uncharacterized for therapeutic potential.

Purpose of the Study:

To investigate the bactericidal activity of HPUFs from Staphylococcus phage Stab21 against E. coli.
To identify potential shared molecular targets between E. coli and Staphylococcus aureus.
To evaluate the utility of Next Generation Sequencing (NGS) for HPUF screening.

Main Methods:

Screening of 96 HPUFs from Stab21 phage against E. coli.
Utilized a Next Generation Sequencing (NGS)-based approach for high-throughput analysis.
Bioinformatic analysis to identify homologous sequences, including homing endonucleases.

Main Results:

Five out of 96 HPUFs exhibited cross-species toxicity against E. coli.
Identified one toxic antibacterial HPUF (toxHPUF) homologous to a homing endonuclease.
Confirmed the effectiveness of NGS in rapidly screening HPUFs for antimicrobial activity.

Conclusions:

Staphylococcus phage HPUFs can possess broad-spectrum bactericidal activity.
The identified toxHPUF suggests potential conserved targets relevant to combating Gram-negative bacteria.
This study validates NGS as an efficient tool for discovering novel phage-derived antimicrobials and advancing phage biology research.