Related Experiment Video
Updated: Jul 21, 2025
13:24
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
11.9K
Applied Molecular-Based Quality Control of Biobanked Samples for Multi-Omics Approach
Anna Michalska-Falkowska1,2, Jacek Niklinski3, Hartmut Juhl2
1Biobank, Medical University of Bialystok, 15-269 Bialystok, Poland.
Cancers
|July 29, 2023
Summary
This study introduces new molecular quality control workflows for biobanking. These methods ensure biospecimens are suitable for advanced omics assays in translational cancer research.
Area of Science:
- Biomedical Sciences
- Translational Research
- Biobanking
Background:
- Biobanks are crucial for high-throughput translational research.
- Novel molecular techniques challenge traditional biobanking practices.
- Standardized quality control is needed for omics assays.
Purpose of the Study:
- To develop and validate molecular quality control workflows for serum/plasma and tissue biospecimens.
- To ensure biospecimen suitability for high-throughput omics assays.
- To create customizable workflows for diverse biobanking settings.
Main Methods:
- Utilized biospecimens from oncological patients.
- Monitored hemolysis and controlled RNA extraction for serum/plasma.
- Performed pathologic evaluation and assessed tumor content for tissue samples.
- Implemented molecular quality control including RNA integrity number and next-generation sequencing (NGS) data analysis.
Main Results:
- Developed distinct preanalytical quality control workflows for serum/plasma and fresh-frozen tissue.
- Established molecular quality control measures aligned with high-throughput assay demands.
- Integrated novel molecular techniques into comprehensive workflows for biobanking.
Conclusions:
- The proposed workflows enhance biospecimen quality for advanced research.
- Molecular quality control is essential for reliable omics data in biobanks.
- These workflows support the integration of innovative techniques in biobanking practices.
