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In Vitro Model of Coronary Angiogenesis
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Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy

Svetlana P Chumakova1,2, Olga I Urazova1,2,3, Vladimir M Shipulin4

  • 1Pathophysiology Division, Siberian State Medical University, Tomsk 634050, Russia.

Biomedicines
|July 29, 2023
PubMed

Insights

Ischemic cardiomyopathy (ICMP) involves angiopoietic endothelial dysfunction, characterized by an imbalance of mediators and altered cell counts. This study explored these changes in patients with coronary heart disease (CHD).

Area of Science:

  • Cardiovascular Research
  • Endothelial Biology
  • Molecular Medicine

Background:

  • Endothelial dysfunction is a hallmark of ischemic cardiomyopathy (ICMP), but its angiopoietic aspects are poorly understood.
  • Investigating endothelial progenitor cells (EPCs) and desquamated endothelial cells (EDCs) is crucial for understanding ICMP pathogenesis.

Purpose of the Study:

  • To identify the imbalance of endothelial dysfunction mediators in patients with coronary heart disease (CHD).
  • To quantify endothelial progenitor cells (EPCs) and desquamated endothelial cells (EDCs) in patients with and without ICMP.

Main Methods:

  • Flow cytometry was used to determine EPCs (CD14+CD34+VEGFR2+) and EDCs (CD45-CD146+).
  • ELISA and immunofluorescence assessed levels of HIF-1α, HIF-2α, ADMA, endothelin-1, angiopoietin-2, MMP-9, galectin-3, VEGF, PDGF, SDF-1, and MCP-1.

Main Results:

  • ICMP patients showed HIF-1α deficiency and HIF-2α excess in vein blood.
  • EDC levels increased in CHD patients, irrespective of ICMP.
  • ICMP patients exhibited elevated galectin-3 in vein blood and increased EDCs, angiopoietin-2, MMP-9, and galectin-3 in sinus blood.

Conclusions:

  • Ischemic cardiomyopathy (ICMP) is associated with angiopoietic endothelial dysfunction.
  • The findings highlight specific molecular and cellular markers indicative of endothelial dysfunction in ICMP.
Abstract

Keywords:
angiogenesiscoronary heart diseaseendothelial progenitor cellsgrowth factorsheart failure

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