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Updated: Jul 21, 2025

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
Pluripotency-Associated microRNAs in Early Vertebrate Embryos and Stem Cells
Pouneh Maraghechi1, Maria Teresa Salinas Aponte1, András Ecker1
1Department of Animal Biotechnology, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences; Agrobiotechnology and Precision Breeding for Food Security National Laboratory, Szent-Györgyi Albert str. 4, 2100 Gödöllő, Hungary.
Abstract:
MicroRNAs (miRNAs), small non-coding RNA molecules, regulate a wide range of critical biological processes, such as proliferation, cell cycle progression, differentiation, survival, and apoptosis, in many cell types. The regulatory functions of miRNAs in embryogenesis and stem cell properties have been extensively investigated since the early years of miRNA discovery. In this review, we will compare and discuss the impact of stem-cell-specific miRNA clusters on the maintenance and regulation of early embryonic development, pluripotency, and self-renewal of embryonic stem cells, particularly in vertebrates.
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