Jove
Visualize
Contact Us

Related Experiment Videos

Aromatic hydroxylations in peroxidations by haemoglobin systems.

L Esclade, D Guillochon, D Thomas

    Xenobiotica; the Fate of Foreign Compounds in Biological Systems
    |July 1, 1986
    PubMed
    Summary

    Haemoglobin catalyzes aromatic hydroxylation of aniline and phenol, forming products whose stability depends on electron donors. Red blood cells enhance product levels and longevity, suggesting implications for drug metabolism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Glycosyl part identified within Balanites aegyptiaca fruit protease.

    International journal of biological macromolecules·2011
    Same author

    Influence of pH on the appearance of active peptides in the course of peptic hydrolysis of bovine haemoglobin.

    Preparative biochemistry & biotechnology·2005
    Same author

    A simple method for the two-step preparation of two pure haemorphins from a total haemoglobin peptic hydrolysate by conventional low-pressure chromatographies.

    Biotechnology and applied biochemistry·2001
    Same author

    Using an experimental design for the optimization of LVV-haemorphin-7 and VV-haemorphin-7 extraction by an organic solvent mixture in the course of bovine haemoglobin peptic hydrolysis.

    Biotechnology and applied biochemistry·2001
    Same author

    Antibacterial activity of a pepsin-derived bovine hemoglobin fragment.

    FEBS letters·2001
    Same author

    Influence of the extent of haemoglobin hydrolysis on the digestive absorption of haem iron in the rat. An in vitro study.

    Experimental physiology·2000
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Biochemistry
    • Pharmacology

    Background:

    • Haemoglobin's catalytic roles are extensively studied, but its activity on aromatic substrates requires further elucidation.
    • Understanding haemoglobin's interactions with xenobiotics is crucial for predicting drug metabolism and toxicity.

    Purpose of the Study:

    • To investigate the catalytic hydroxylation of aromatic compounds by haemoglobin.
    • To explore the influence of electron donors and red blood cell environment on these reactions.

    Main Methods:

    • Studied haemoglobin-catalyzed reactions using three systems: NADH-methylene blue-haemoglobin, ascorbic acid-haemoglobin, and intact red blood cells.
    • Analyzed the hydroxylation of aromatic substrates like aniline and phenol, and monitored product stability.

    Main Results:

    Related Experiment Videos

    • Haemoglobin hydroxylated aniline and phenol, but not acetanilide or p-toluidine; dealkylations were not observed.
    • Hydroxylation reactions are proposed as intermediates in haemoglobin-catalyzed peroxidations.
    • Product lifetime was significantly extended in the presence of electron donors (NADH, ascorbic acid) and within red blood cells due to endogenous electron donor recycling.

    Conclusions:

    • Haemoglobin exhibits specific aromatic hydroxylation activity, particularly on aniline and phenol.
    • The presence and recycling of electron donors, as seen in red blood cells, are critical for stabilizing reaction products.
    • These findings highlight potential implications for haemoglobin's role in drug metabolism due to its abundance in the body.