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Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
Published on: August 12, 2009
Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii
Federico Perozeni1, Thomas Baier2
1Department of Biotechnology, University of Verona, 37134 Verona, Italy.
Abstract:
The green model microalga Chlamydomonas reinhardtii recently emerged as a sustainable production chassis for the efficient biosynthesis of recombinant proteins and high-value metabolites. Its capacity for scalable, rapid and light-driven growth in minimal salt solutions, its simplicity for genetic manipulation and its "Generally Recognized As Safe" (GRAS) status are key features for its application in industrial biotechnology. Although nuclear transformation has typically resulted in limited transgene expression levels, recent developments now allow the design of powerful and innovative bioproduction concepts. In this review, we summarize the main obstacles to genetic engineering in C. reinhardtii and describe all essential aspects in sequence adaption and vector design to enable sufficient transgene expression from the nuclear genome. Several biotechnological examples of successful engineering serve as blueprints for the future establishment of C. reinhardtii as a green cell factory.
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