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Related Experiment Video

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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

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Published on: April 8, 2009

Monkeypox: A Histopathological and Transmission Electron Microscopy Study.

Chiara Moltrasio¹, Francesca Laura Boggio², Maurizio Romagnuolo^1,3

¹Dermatology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy.

|July 29, 2023

Summary

Histopathological analysis of skin biopsies revealed characteristic changes in human monkeypox virus (hMPXV) infections, including epidermal necrosis and specific cytopathic effects in keratinocytes. This aids in diagnosing mpox and understanding Orthopoxvirus infections.

Keywords:

cutaneous manifestations histopathology monkeypox transmission electron microscopy virology

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Area of Science:

Dermatopathology
Virology
Microscopy

Background:

The 2022 human monkeypox virus (hMPXV) outbreak emphasized the diagnostic value of skin lesions.
Dermatopathology is crucial for diagnosing hMPXV infections.

Purpose of the Study:

To analyze histopathological and microscopical findings in cutaneous lesions of hMPXV infection.
To expand knowledge of hMPXV and other Orthopoxvirus infections.

Main Methods:

Analysis of skin biopsies from patients with confirmed hMPXV infection.
Histopathological examination and transmission electron microscopy.

Main Results:

Prominent findings included epidermal necrosis with "shadow cells" and affected hair follicles/acrosyringium.
Cytopathic effects observed: ballooning keratinocytes, Guarnieri bodies, nuclear "ground glass" appearance, and neutrophil exocytosis.
Viral particles were found in keratinocyte cytoplasm and, to a lesser extent, in mesenchymal cells.

Conclusions:

Histopathological findings provide key insights into hMPXV cutaneous manifestations.
This study enhances understanding of hMPXV and potential future Orthopoxvirus trends.