Plasma hemoglobin determination by recording derivative spectrophotometry.

Area of Science:

• Analytical Chemistry
• Biochemistry

Background:

• Derivative spectrophotometry (DS) is a mathematical technique used to analyze absorbance curves.
• It enhances rapid changes and suppresses slow changes in spectral data.
• Accurate plasma hemoglobin determination is crucial in clinical diagnostics.

Purpose of the Study:

• To present a simple, rapid, and reproducible method for determining plasma hemoglobin concentration.
• To demonstrate the efficacy of DS in analyzing plasma samples.
• To validate a method that is robust against common interfering substances.

Main Methods:

• Utilized recording derivative spectrophotometry (DS) to analyze plasma samples.
• Measured the derivative (slope) of absorbance curves.
• Developed a physicomathematic approach for hemoglobin quantification.

Main Results:

• The DS method accurately determined plasma hemoglobin concentration.
• The method proved effective even with interfering substances like bilirubin and myoglobin.
• The analysis showed robustness against marked turbidity.
• No toxic reagents were employed in the procedure.

Conclusions:

• Derivative spectrophotometry provides a reliable and non-destructive method for plasma hemoglobin analysis.
• The developed technique is simple, rapid, and reproducible.
• This method allows for subsequent analyses of the same sample, offering significant advantages in clinical settings.