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Updated: Jun 20, 2026
A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Published on: January 1, 2016
Development of a green liquid-phase microextraction procedure using a customized device for the comprehensive
Madson M Nascimento1, Jeancarlo P Dos Anjos2, Melise L Nascimento3
1Centro Universitário SENAI-CIMATEC, Av. Orlando Gomes, 1845 - Piatã, 41650-010, Salvador, BA, Brazil; Instituto Nacional de Ciência e Tecnologia Em Energia e Ambiente - INCT E&A, Universidade Federal da Bahia, 40170-115, Salvador, BA, Brazil; Centro Interdisciplinar de Energia e Ambiente - CIEnAm, Universidade Federal da Bahia, 40170-115, Salvador, BA, Brazil.
Abstract:
In this work, we reported the development of a novel, simple, and green liquid-phase microextraction (LPME) procedure based on the use of a customized device for the determination of 47 multiclass pesticides in red, white, and rosè wine samples by GC-MS. The main parameters that affect the LPME were optimized using multivariate statistical techniques such as centroid-simplex mixture design and Doehlert design. The optimal conditions were: 70 μL of toluene as extractor solvent; concentration of NaCl (2.7%, m v-1); pH 4; and an extraction time of 30 min, under vortex-assisted agitation (at 500 rpm). After validation, it was possible to obtain LOQ values as low as 7.63 ng L-1 and extraction recoveries ranging from 81.7% to 119% for most of the target pesticides. The application of exploratory analysis, specifically Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), provided evidence indicating contamination in the different types of wine samples, primarily by systemic fungicides.
