Real-time monitoring ATP variation in human cancer organoids for a long term by DNA-based nanosensor

Area of Science:

• Biomedical Engineering
• Cancer Research
• Nanotechnology

Background:

• Cancer organoids are valuable for predicting drug responses.
• Adenosine triphosphate (ATP) detection is crucial for assessing organoid growth and drug efficacy.
• Current ATP detection methods are limited to single time points, hindering comprehensive analysis.

Purpose of the Study:

• To develop a novel DNA-based ATP nanosensor for real-time, long-term monitoring of ATP levels in cancer organoids.
• To overcome the limitations of current single-time-point ATP detection methods.
• To enable continuous monitoring of organoid growth status and drug responses.

Main Methods:

• Development of a DNA-based nanosensor utilizing a tetrahedral DNA framework for enhanced cell permeability and intracellular detection.
• Incorporation of ATP-mediated molecular replacement for sensitive and selective ATP recognition.
• Real-time ATP monitoring in human lung cancer organoids for up to 26 days.

Main Results:

• The developed DNA-based ATP nanosensor demonstrated high biocompatibility and low cytotoxicity.
• The nanosensor achieved real-time intracellular ATP detection within organoids.
• Successfully generated growth status curves for organoids over a 26-day period during drug sensitivity testing.

Conclusions:

• The DNA-based ATP nanosensor offers a feasible solution for real-time, long-term ATP monitoring in cancer organoids.
• This technology overcomes the clinical dilemma of selecting optimal time points for ATP detection.
• Enables comprehensive assessment of organoid growth and drug responses, advancing personalized cancer therapy.