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An image-guided microfluidic system for single-cell lineage tracking.

Mahmut Aslan Kamil1, Camille Fourneaux2, Alperen Yilmaz3

  • 1Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, Switzerland.

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|August 1, 2023
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Summary
This summary is machine-generated.

This study introduces a microfluidic system for precise cell lineage tracking over multiple generations. The technology enables single-cell isolation for downstream transcriptome analysis, advancing cell biology research.

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Area of Science:

  • Cell Biology
  • Bioengineering
  • Genomics

Background:

  • Cell lineage tracking is crucial for understanding development and disease but remains a significant challenge in biology.
  • Microfluidic technologies offer precise control over single cells, presenting a promising solution for lineage analysis.

Purpose of the Study:

  • To develop and validate a valve-based microfluidic system for tracking single-cell lineage over multiple generations.
  • To enable downstream molecular analysis of isolated sister cells.

Main Methods:

  • A microfluidic device was designed to trap single cells in growth chambers for division monitoring.
  • Automated image processing controlled cell loading, growth, and division monitoring.
  • Sister cells were isolated after a user-defined number of divisions and extracted for analysis.

Main Results:

  • The microfluidic system successfully tracked chicken erythroleukemia and progenitor cells over two generations with >90% viability.
  • Sister cells were efficiently isolated and extracted in a 500 nL volume.
  • The extracted cell volumes were compatible with single-cell RNA sequencing.

Conclusions:

  • The developed microfluidic platform provides a robust method for multi-generational cell lineage tracking.
  • This technology facilitates the study of single-cell decision-making and enables subsequent transcriptome analysis.