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Recent progress of SELEX methods for screening nucleic acid aptamers
Chao Zhu1, Ziru Feng1, Hongwei Qin1
1Institute of Quality Standard and Testing Technology for Agro-products, Shandong Academy of Agricultural Sciences, Jinan, 250100, China; Shandong Provincial Key Laboratory Test Technology on Food Quality and Safety, Jinan, 250100, China.
Talanta
|August 1, 2023
Summary
Nucleic acid aptamers, developed via Systematic Evolution of Ligands by Exponential Enrichment (SELEX), offer versatile applications. Recent SELEX advancements enhance aptamer performance for disease diagnosis and drug discovery.
Area of Science:
- Biotechnology
- Molecular Biology
- Chemical Biology
Background:
- Aptamers are oligonucleotide sequences with high affinity and specificity for targets, acting as
Purpose of the Study:
- To review novel and improved Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methods developed in the past five years.
- To highlight advancements in aptamer screening strategies, carrier materials, and technical improvements.
- To discuss challenges and future directions for SELEX and aptamer applications.
Main Methods:
- Review of recent literature on SELEX methodology advancements.
- Analysis of SELEX improvements in carrier materials, technical aspects, and real-sample screening.
- Discussion of post-SELEX procedures and screening strategies.
Main Results:
- SELEX methods have evolved significantly over 32 years, with recent innovations focusing on improved sequence performance and screening mechanisms.
- Progress has been made in areas such as carrier materials, technical improvements, real-sample screening, and post-SELEX processing.
- Various SELEX strategies, implementation features, and screening parameters have been refined.
Conclusions:
- Recent advancements in SELEX methods offer enhanced aptamer performance and broader application potential.
- Addressing current challenges in SELEX is crucial for the successful practical application of aptamers.
- Future directions involve developing more convenient, efficient, and stable SELEX methods.
