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Optimization of a Validated Protocol That Determines Bioavailable Fluoride in Toothpastes
Luis Fernando Bandeira Miranda1, Cinthia Pereira Machado Tabchoury1, Jaime Aparecido Cury1
1Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.
Abstract:
A validated protocol used to determine how much of total fluoride is bioavailable in toothpaste formulations was evaluated if it could be simplified regarding the following parameters: (1) concentration of the slurry (0.25-4%), (2) centrifugation speed (1,000-12,000 g) and time (1-10 min), and (3) incubation time for FPO32- ion hydrolysis and insoluble-F dissolution (15-60 min). Fresh and aged Na2FPO3/CaCO3 and fresh NaF/SiO2-based toothpastes were analyzed (n = 9). The improvements were the centrifugation at 1,000 g for 5 min is enough to separate insoluble-F and the time for FPO32- ion hydrolysis and insoluble-F dissolution can be shortened to 30 min.
