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Short heat shock has a long-term effect on mesenchymal stem cells' transcriptome.
Ivana Ribarski-Chorev1, Gisele Schudy1, Carmit Strauss1
1The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.
Iscience
|August 2, 2023
Summary
Heat stress (HS) alters mesenchymal stem cell (MSC) identity through persistent changes in gene expression. Histone modifiers may explain how short-term HS causes long-term effects on cell identity.
Area of Science:
- Cell Biology
- Molecular Biology
- Genomics
Background:
- Heat stress (HS) poses significant risks to physiological systems.
- The molecular mechanisms underlying HS effects, particularly on stem cells, are not fully understood.
Purpose of the Study:
- To investigate the impact of HS on mesenchymal stem cells (MSCs).
- To elucidate the molecular mechanisms driving HS-induced alterations in MSC identity.
Main Methods:
- Transcriptome analysis of MSCs exposed to short-term HS.
- Comparison of gene expression profiles before, during, and after HS recovery.
- Analysis of bivalent gene activation and histone modifier expression.
Main Results:
- HS significantly altered the MSC transcriptional landscape.
- Persistent changes in cell identity were observed even after short HS exposure.
- Transient upregulation of MLL and histone modifiers correlated with stable bivalent gene activation.
Conclusions:
- Short-term HS can induce stable, long-term changes in MSC identity.
- Histone modifiers may mediate the persistent effects of HS on cell identity.
- Findings offer potential strategies to predict and modify HS-induced cellular changes.
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