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Ononin Relieves the Thyroid Cancer Progression through Targeting the Caspase 3 and CD274 Expression Levels

Liangliang Du1, Douyun Zeng1, Xian Hu1

  • 1Department of Endocrinology, Yueyang Hospital of Traditional Chinese Medicine.

Biological & Pharmaceutical Bulletin
|August 2, 2023
PubMed

Insights

Ononin, a natural compound, inhibits thyroid cancer (TC) cell growth and promotes apoptosis. It regulates caspase 3 (CASP3) and CD274 expression, offering a potential therapeutic strategy for thyroid cancer.

Area of Science:

  • Endocrinology
  • Oncology
  • Pharmacology

Background:

  • Thyroid cancer (TC) is the most prevalent endocrine malignancy.
  • Ononin, an isoflavone, possesses antioxidant and anti-inflammatory properties.
  • Understanding ononin's role in TC progression is crucial for developing new treatments.

Purpose of the Study:

  • To investigate the anti-cancer effects of ononin on thyroid cancer cells.
  • To elucidate the molecular mechanisms underlying ononin's action in TC.

Main Methods:

  • Cell viability was assessed using the cell counting kit-8 (CCK8) assay.
  • Apoptosis and cell death were analyzed via TUNEL staining and flow cytometry.
  • Gene and protein expression levels of CASP3 and CD274 were determined using qRT-PCR and Western blot.

Main Results:

  • Ononin significantly reduced TC cell viability and induced apoptosis.
  • Ononin treatment decreased CD274 expression and increased CASP3 expression in TC cells.
  • Ononin enhanced T cell cytotoxicity and its effects were potentiated by CASP3 overexpression or CD274 knockdown.

Conclusions:

  • Ononin exhibits anti-thyroid cancer activity by inhibiting cell proliferation and inducing apoptosis.
  • Ononin exerts its effects through the regulation of CASP3 and CD274 expression.
  • Ononin represents a promising therapeutic candidate for thyroid cancer treatment.
Keywords:
CD274caspase 3ononinthyroid cancer

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