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MiR-181c-5p Regulates Lung Adenocarcinoma Progression via Targeting PRKN

Jing Wang1, Man Li2, Meigui Wang3

  • 1Department of Oncology, The Second People's Hospital of Liaocheng, Linqing, 252600, Shandong Province, China.

Biochemical Genetics
|August 2, 2023
PubMed

Insights

MicroRNA miR-181c-5p is elevated in lung adenocarcinoma and drives cancer progression by targeting the Parkin gene (PRKN). Inhibiting miR-181c-5p suppressed tumor growth, suggesting its potential as a biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play a critical role in the development of lung adenocarcinoma.
  • The specific role and mechanism of miR-181c-5p in lung adenocarcinoma are not well understood.

Purpose of the Study:

  • To investigate the regulatory mechanism of miR-181c-5p in lung adenocarcinoma progression.
  • To explore the potential of miR-181c-5p as a diagnostic and predictive marker.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to measure miR-181c-5p levels.
  • Cell viability, migration, and invasion assays (CCK-8, Transwell).
  • Bioinformatic prediction (miRDB) and dual-luciferase reporter assay to confirm target gene (PRKN).

Main Results:

  • miR-181c-5p expression was significantly upregulated in lung adenocarcinoma tissues and cells.
  • Downregulation of miR-181c-5p inhibited cell viability, migration, and invasion.
  • PRKN was identified as a direct target of miR-181c-5p, with inverse correlation in expression.
  • PRKN mediated the effects of miR-181c-5p on lung adenocarcinoma progression.

Conclusions:

  • miR-181c-5p promotes lung adenocarcinoma progression by targeting PRKN.
  • miR-181c-5p represents a potential diagnostic and predictive biomarker for lung adenocarcinoma.
  • Targeting miR-181c-5p may offer novel therapeutic strategies for lung adenocarcinoma.
Keywords:
InvasionMicroRNAsMigrationViability

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