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Updated: Jul 20, 2025

Dissection of Larval Zebrafish Gonadal Tissue
Published on: April 26, 2017
The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn
Jing-Yu Luo1, Shuai-Qi Shen1,2, Hai-Jing Xu1
1College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, People's Republic of China.
Abstract:
Some Zinc finger (ZnF) proteins are required for masculinization in silkworms. In the present study, a masculinizer gene (Mr-Masc) with multi-tissue expression is identified in the freshwater prawn Macrobrachium rosenbergii. The Mr-Masc is clustered into a separate branch with ZnF proteins from decapoda by phylogenetic tree analysis. Moreover, Mr-Masc silencing in male postlarvae prawn results in functional sex reversal females known as neo-females, which are applied to all-male monosex offspring breeding. This manipulation has been significant in sexually dimorphic cultured species. In addition, several significantly expressed transcripts are enriched and the effects of crucial signal pathways are focused through the comparative transcriptomic analysis in Mr-Masc gene knockdown. The significantly differentially expressed epidermal growth factor, upregulated low-density lipoprotein receptor, flotillin, and sex-lethal unigenes, downregulated heat shock proteins and forkhead box homologs are focused. The finding offers an innovative perspective on Masc proteins' evolution and physiological function.
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