Related Experiment Video
Updated: Jul 20, 2025
Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
Published on: September 26, 2019
Quantifying the mycobiome and its major constituents on the skin of 20 normal dogs
Richard G Harvey1, David Duclos2, Janina Krumbeck3,4
1Willows Referral Services (part of Linnaeus Veterinary Ltd), Shirley, Solihull, UK.
Objective:
To report the density, and constituents, of the mycobiome on the skin surface of normal dogs.
Animals:
20 normal dogs were recruited for this study, with informed consent in all cases.
Methods:
Flocked swabs were used to sample the skin surface and to sample the skin surface after superficial scraping with a blunted scapula. Both samples were taken within a brass guide with an internal area of 3.5 cm-2. Next-generation DNA sequencing was used to identify and quantify components of the mycobiome.
Results:
The median density of the mycobiome was 1.1 X 105 cm-2 (IQR, 27,561, 409,572). Cladosporium spp and Vishniacozyma victoriae were found on all 20 dogs.
Clinical Relevance:
Knowledge of the density and the composition of the cutaneous mycobiome will increase our understanding of skin biology and may have relevance to future therapeutic trials.
