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PROBFIND: a computer program for selecting oligonucleotide probes from peptide sequences.

R M Lewis

    Nucleic Acids Research
    |January 10, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    PROBFIND is a computer program that helps researchers select synthetic oligonucleotide probes for screening genetic libraries. It efficiently identifies potential probes based on user-defined criteria, simplifying molecular biology workflows.

    Area of Science:

    • Molecular Biology
    • Bioinformatics
    • Computational Biology

    Background:

    • Synthetic oligonucleotides are essential tools for screening cDNA and genomic libraries.
    • Accurate selection of these probes is crucial for efficient library screening.

    Purpose of the Study:

    • To introduce PROBFIND, a computer program designed to facilitate the selection of synthetic oligonucleotide probes.
    • To provide researchers with an efficient and user-friendly tool for identifying optimal probes.

    Main Methods:

    • The PROBFIND program accepts amino acid sequences as input.
    • Users define minimum probe length and maximum degeneracy parameters.
    • The program interactively generates a list of potential probes and their corresponding protein locations.

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    Main Results:

    • PROBFIND successfully identifies synthetic oligonucleotide probes meeting user-defined specifications.
    • The program offers interactive modification of probe length and degeneracy criteria.
    • It provides output to both screen and file for user convenience.

    Conclusions:

    • PROBFIND simplifies and enhances the accuracy of synthetic oligonucleotide probe selection.
    • Its ease of use and adaptability make it suitable for various microcomputer systems.
    • The program streamlines the process of screening cDNA and genomic libraries.